Figure 8. Overexpression of PKMYT1 in renal cancer cells and tissues. The expression of PKMYT1 in renal cancer cells was measured using real-time quantitative reverse transcription-PCR (A) and western blotting assays (B). The expression of PKMYT1 in renal cancer tissues and paired normal tissues was measured using real-time quantitative reverse transcription-PCR (C), western blotting assays (D) and immunohistochemistry (E). Data are presented as the mean ± standard deviation of three independent experiments, and were compared to HK-2 cells. **P < 0.01, ***P < 0.001; ****P < 0.0001.