Research Paper Volume 13, Issue 23 pp 25291—25303
Sorbitol accumulation decreases oocyte quality in aged mice by altering the intracellular redox balance
Figure 4. Effects of sorbitol accumulation on the levels of ROS and GSH and the expression of SOD1 in IVM oocytes. (A) The levels of ROS in young IVM oocytes (young), aged IVM oocytes (aged), and aged IVM oocytes treated with sorbinil (sorbinil). Fifteen oocytes in each group from three independent experiments were tested. (B) The differences in ROS levels in the above three groups. Fifteen oocytes in each group from three independent experiments were tested. (C) The levels of GSH in above three groups. (D) The differences in GSH levels in the above three groups. (E) The expression of SOD1 in the IVM oocytes. All experiments were repeated at least three times. Scale bar = 50 μm, *P < 0.05, **P < 0.01.