Research Paper
A novel circular RNA DNAH1 inhibits osteosarcoma progression by interacting with miR-663a
Figure 6. Over-expression of circ-DNAH1 reverses miR-663a-induced enhancement of cell proliferation, migration and invasion in OS cells. (A) qRT-PCR quantification of miR-663a in MG63 and U2OS cells transfected with circ-DNAH1 or siRNAs against circ-DNAH1 (si-circ-DNAH1). (B) Spearman correlation analysis of the circ-DNAH1 and miR-663a levels in 60 cases of OS patients, (C) CCK-8 cell proliferation assay of MG63 and U2OS cells expressing miR-663a mimics or miR-663a mimics in combination with circ-DNAH1. (D) Transwell migration assays of MG63 and U2OS cells expressing miR-663a mimics or miR-663a mimics in combination with circ-DNAH1. (E) Transwell invasion assays of MG63 and U2OS cells expressing miR-663a mimics or miR-663a mimics in combination with circ-DNAH1. (F) Immunoblotting analysis of EMP3, MEK and ERK, as well as pMEK, pERK and p-c-Raf in MG63 cells expressing miR-663a mimics or miR-663a mimics in combination with circ-DNAH1. GAPDH serves as a loading control. miR-NC: negative controls for miR-663a mimics. Data are shown as mean ± SD. **P < 0.01 and ns = no significance.