Research Paper
A novel circular RNA DNAH1 inhibits osteosarcoma progression by interacting with miR-663a
Figure 5. miR-663a is up-regulated in OS tissues and cell lines, and promotes OS progression through targeting EMP3 in vitro. (A) qPCR analysis of miR-663a expression in OS tumor tissues and the adjacent noncancerous tissues (n = 60). (B) qPCR dialysis of miR-663a expression in HOS, 143B, Saos2, MG63, U2OS and hFOB1.19 cells. (C) The Kaplan-Meier analysis of disease-free survival of the low and high miR-663a expression groups. (D, E) CCK-8 cell proliferation assay of MG63 and U2OS cells expressing miR-663a mimics or miR-663a inhibitor. (F, G) Transwell migration assays of MG63 and U2OS cells expressing miR-663a mimics or miR-663a inhibitor. (H, I) Transwell invasion assays of MG63 and U2OS cells expressing miR-663a mimics or miR-663a inhibitor. (J, K) Immunoblotting analysis of EMP3, MEK and ERK, as well as pMEK, pERK and p-c-Raf in MG63 and U2OS cells expressing miR-663a mimics or miR-663a inhibitor. GAPDH serves as a loading control. miR-NC and inh-NC: negative controls for miR-663a mimics and miR- 663a inhibitor, respectively. Data are shown as mean ± SD. **P < 0.01 and ns = no significance.