Research Paper

Figure 3. Circ-DNAH1 inhibits proliferation, migration and invasion of OS cells in vitro. (A, B) qRT-PCR analysis of circ-DNAH1 and DNAH1 mRNA expression in MG63 and U2OS cells infected with recombinant lentivirus, LV-circ-DNAH1 (A) or LV-circ-DNAH1 shRNA-1/2 (B). (C, D) The MG63 and U2OS cells were infected with recombinant lentivirus, LV-circ-DNAH1 (C) or LV-circ-DNAH1 shRNA-1/2 (D), and cell proliferation was measured at Oh, 24h, 48h and 72h. (E, F) Transwell migration assay of MG63 and U2OS cells infected with recombinant lentivirus, LV-circ-DNAH1 (E) or LV-circ- DNAH1 shRNA-1/2 (F). (G, H) In vitro scratch migration assay of MG63 and U2OS cells infected with recombinant lentivirus, LV-circ-DNAH1 (G) or LV-circ-DNAH1 shRNA-1/2 (H). (I, J) Transwell invasion assay of MG63 and U2OS cells infected with recombinant lentivirus, LV-circ- DNAH1 (I) or LV-circ-DNAH1 shRNA-1/2 (J). Data are shown as mean ± SD. **P < 0.01 and ns = no significance.