Research Paper
A novel circular RNA DNAH1 inhibits osteosarcoma progression by interacting with miR-663a
Figure 1. hsa_circRNA_104892 expression is significantly downregulated in OS and decreased circ- hsa_circRNA_104892 expression predicts poor prognosis. (A) Hierarchical clustering heat map showing the top 250 differentially expressed circular RNAs in OS cell lines versus human osteosarcoma in the order of significance based on P value, with hsa_circRNA_l04892 on the very top. Data is retrieved from the GEO database (GSE96964). (B) qRT-PCR analysis of hsa_circRNA_l04892 expression in HOS, 143B, Saos2, MG63, U2OS and hFOB1.19 cells. Data we shown as mean ± SD. **P < 0.01. (C) qRT-PCR analysis of has_circRNA_l04892 expression from 60 OS tumor tissues paired with the adjacent noncancerous tissues. (D) Kaplan-Meier analysis of the survival rate of OS patients with low and high hsa_circRNA_l04892 expression (n = 30 in each group). Data are shown as mean ± SD. ***P < 0.001.