Research Paper Volume 13, Issue 20 pp 23620—23636

Figure 5. Nmnat2 up-regulates AMPK in N2a/APPswe cells. (A1–A4ʹ) N2a/APPswe cells were transfected with the empty vector (A1–A4) or Flag-Nmnat2 (Nmnat2) (A1ʹ–A4ʹ) for forty eight hours, and then AMPK was detected by immunofluorescence (IF), Nmnat2 over-expressing increased the fluorescence intensity of AMPK as shown by the white arrow (A1ʹ–A4ʹ). (B1–B4ʹ) N2a/APPswe cells were transfected with the scrambled shRNA control (Vector) (B1–B4) or shRNA-Nmnat2 (siNmnat2) (B1ʹ–B4ʹ) for forty eight hours, and then AMPK was detected by IF, knockdown of Nmnat2 decreased the fluorescence intensity of AMPK as shown by the white arrow (B1ʹ–B4ʹ). The data were representative of at least three independent experiments.