Research Paper Volume 13, Issue 20 pp 23620—23636

Nmnat2 attenuates amyloidogenesis and up-regulates ADAM10 in AMPK activity-dependent manner

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Figure 5. Nmnat2 up-regulates AMPK in N2a/APPswe cells. (A1A4ʹ) N2a/APPswe cells were transfected with the empty vector (A1A4) or Flag-Nmnat2 (Nmnat2) (A1ʹA4ʹ) for forty eight hours, and then AMPK was detected by immunofluorescence (IF), Nmnat2 over-expressing increased the fluorescence intensity of AMPK as shown by the white arrow (A1ʹA4ʹ). (B1B4ʹ) N2a/APPswe cells were transfected with the scrambled shRNA control (Vector) (B1B4) or shRNA-Nmnat2 (siNmnat2) (B1ʹB4ʹ) for forty eight hours, and then AMPK was detected by IF, knockdown of Nmnat2 decreased the fluorescence intensity of AMPK as shown by the white arrow (B1ʹB4ʹ). The data were representative of at least three independent experiments.