Research Paper Volume 13, Issue 20 pp 23620—23636

Figure 4. Nmnat2 enhances expression of AMPK and p-AMPK. (A, B) The cortex extracts were prepared from 10 months old Tg2576 mice (Tg) and the age-matched littermates (Non-Tg), then Nmnat2, AMPK and p-AMPK were measured by western blot (A) and quantitative analysis (B) (n=5 for each group). The data were expressed as means ± S.D. *P < 0.05, **P < 0.01 vs control. (C, D) N2a/APPswe cells were transfected with Flag-Nmnat2 (Nmnat2) or the empty vector for forty eight hours, and then Nmnat2, AMPK and p-AMPK were detected by western blot (C), quantitative analysis (D). (E, F) N2a/APPswe cells were transfected with shRNA-Nmnat2 (siNmnat2) or the scrambled shRNA control (Vector) for forty eight hours, and then Nmnat2, AMPK and p-AMPK were also detected by western blot (E), quantitative analysis (F). The data were representative of at least three independent experiments and expressed as means ± S.D.. *P < 0.05, **P < 0.01 vs control.