Research Paper

Figure 7. LINC01224 regulates the proliferation, cycle and apoptosis by competing with YES1 for binding to miR-193a-5p. (A) CCK8 showed that the miR-193a-5p inhibitor, YES1-overexpressing plasmid, and wt-LINC01224 plasmid partially reversed the inhibitory effect of silencing of LINC01224 on the proliferation of AGS cells, whereas the mut-LINC01224 plasmid could not. (B, C) Colony formation assays showed that the miR-193a-5p inhibitor, YES1-overexpressing plasmid, and wt-LINC01224 plasmid attenuated the growth arrest of AGS cells caused by the knockdown of LINC01224, while the mut-LINC01224 plasmid could not. (D, E) EDU experiments showed that the miR-193a-5p inhibitor, YES1-overexpressing plasmid, and wt-LINC01224 plasmid attenuated the growth arrest of AGS cells caused by the knockdown of LINC01224, while the mut-LINC01224 plasmid could not. Scale bars = 50 μm. (F, G) Flow cytometry assays showed that the miR-193a-5p inhibitor, YES1-overexpressing plasmid, and wt-LINC01224 plasmid partially reversed the apoptosis of AGS cells caused by the knockdown of LINC01224, whereas the mut-LINC01224 plasmid could not. (H, I) Flow cytometry assays showed that the miR-193a-5p inhibitor, YES1-overexpressing plasmid, and wt-LINC01224 plasmid partially reversed the G1 phase cell cycle arrest of AGS cells caused by the knockdown of LINC01224, whereas the mut-LINC01224 plasmid could not.