Research Paper

Figure 3. Effect of LINC01224 on the biological behavior of gastric cancer cells in vitro. (A) LINC01224 expression was knocked down by two shRNAs targeting LINC01224 in SNU-1 and AGS cells. (B) CCK-8 assay found that knockdown of LINC01224 significantly decreased the proliferation of AGS and SNU-1 cells compared with sh-control. (C) The colony numbers of SNU-1 and AGS cells transfected with sh-LINC01224 were significantly lower than those transfected with sh-control. (D) EdU assay showed that knockdown of LINC01224 inhibited the proliferation of SNU-1 and AGS cells. Scale bars = 50 μm. (E) Flow cytometry assay found that knockdown of LINC01224 promoted apoptosis of SNU-1 and AGS cells by Annexin V-PI staining. (F) Flow cytometry analysis of cell cycle distribution showed that the number of SNU-1 and AGS cells arrested in the G1 phase was significantly increased after knockdown of LINC01224.