This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 13, Issue 18 pp 22459—22473
miR-19b-3p relieves intervertebral disc degeneration through modulating PTEN/PI3K/Akt/mTOR signaling pathway
Figure 5. Inhibiting PTEN enhanced the protective effect of miR-19b-3p. (A) Detection of miR-19b-3p targets was made using the database ENCORI (http://starbase.sysu.edu.cn). (B) The database mirPath v.3 (http://snf-515788.vm.okeanos.grnet.gr) was applied to query the downstream pathway of miR-19b-3p. (C) Venn diagram of 217 targets of miR-19b-3p and the mTOR signaling pathway. (D) Base complementary sequences of miR-19b-3p and PTEN. 293-T cells were transfected with miR-19b-3p mimics for 24 hours. (E) The dual-luciferase reporter assay was implemented for ascertaining the binding relationship between miR-19b-3p and PTEN. HNPCs were transfected with miR-19b-3p mimics for 24 hours. (F–H) The PTEN profile in HNPCs was measured by qRT-PCR, WB and cellular immunofluorescence. nsP>0.05,* P <0.05, ** P <0.01 (vs.miR-NC group) N=3.