Research Paper Volume 13, Issue 18 pp 22432—22443

Figure 4. miR-422a directly targets PLP2. (A) The putative binding sequence of miR-422a of 3’UTR of PLP2. The prediction was obtained from StarBase 2.0. (B) Luciferase reporter assay in HEK293T cells co-transfected with miR-422a mimics or mimics a negative control plus reporter vector containing wild-type or mutant miR-422a binding sequence 3′-UTR of PLP2. (C) The mRNA expression of PLP2 in SKOV3 and control cells. (D) The mRNA expression of PLP2 in SKOV3 cells with Foxo3 knockdown or overexpressing. (E) Transfection efficiency in miR-422a-knockdown or miR-422a-overexpressing SKOV3 cells. (F) The mRNA expression of PLP2 in SKOV3 cells with miR-422a knockdown or overexpressing. (G) The mRNA expression of PLP2 in SKOV3 cells co-transfected with Foxo3-overexpressing and miR-422a-overexpressing vector. (H) The protein expression of PLP2 in SKOV3 and control cells. (I) The protein expression of PLP2 in SKOV3 cells with Foxo3 knockdown or overexpressing. (J) The protein expression of PLP2 in SKOV3 cells with miR-422a knockdown or overexpressing. Data were expressed as means ± standard deviation. For each treatment, three replicates were used. ^**p < 0.01, ^***p < 0.001.