Figure 5. I/R induced up-regulation of Cx32 expression and activation of autophagy. (A) Double labeling of Cx32 and β-tubulin in the brains of Sham or I/R group. Scale bars, 50 μm. (B) Images acquired by transmission electron microscope. The arrow marks autophagic vacuoles. (C–E) Representative bands of autophagy-related proteins after I/R. Variation in protein loading was determined by blotting with an anti-β-actin antibody.