Research Paper Volume 13, Issue 17 pp 21743—21757
lncRNA HCG11 suppresses human osteosarcoma growth through upregulating p27 Kip1
Figure 6. p27 Kip1 is a key effector for HCG11 exerting biological functions. (A) Overexpression and silence of p27 Kip1 in OS cells validated by qRT-PCR. (B, C) Influence of HCG11 and p27 Kip1 on the proliferation OS cells was assessed by CCK8 assays. (D, E) Influence of HCG11 and p27 Kip1 on the cell cycle of OS cells was analyzed by flow cytometry. (F, G) Influence of HCG11 and p27 Kip1 on the DNA replication of OS cells was assessed by EdU assay. *P < 0.05, **P < 0.01.