Research Paper Volume 13, Issue 17 pp 21571—21586

TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages

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Figure 3. Knockdown of TNFSF9 inhibits the migration and invasion of pancreatic cancer cells. (A–D) Transwell experiment was used to analyze the migration and invasion of BXPC-3 and PANC-1 cells after TNFSF9 knockdown. (E, F) The cell scratch test was used to analyze the migration of BXPC-3 and PANC-1 cells after TNFSF9 knockdown. (G–J) Western blots were used to analyze the expression levels of EMT-associated proteins; Vimentin, N-cadherin and E-cadherin in BXPC-3 and PANC-1 cells after knockdown of TNFSF9. *P < 0.05, **P < 0.01, and ***P < 0.001.