Research Paper Volume 13, Issue 17 pp 20962—20991

Figure 2. (A) Effect of glutamine deprivation on cell viability. MCF-7 and A549 cells were grown for 72 hours in either high-glucose (high) or low-glucose (low) medium, in the presence (+Gln) or in the absence (−Gln) of 2 mM glutamine or in the presence of indicated concentrations of 2-deoxyglucose (2DG). Cells were stained with trypan blue and counted. The percentage of dead, trypan blue-positive cells is shown. Data are mean ± S.D. of three independent experiments. (B) The effect of glutamine deprivation on TIS escape. Doxorubicin-induced senescent MCF-7 and A549 cells were grown in high-glucose (high) or low-glucose (low) medium, in the presence (+Gln) or in the absence (−Gln) of 2 mM glutamine or in the presence of 2-deoxyglucose (2DG). Colonies that evaded the senescent growth arrest were stained and counted. Left panels: quantification of the colony escape assay. Data are mean ± S.D. of three independent experiments. Right panels: representative images of colony escape assay.