Research Paper Volume 13, Issue 17 pp 21526—21546

Figure 4. Liver functions affected by the dietary interventions. (A) Representative images of liver sections of mice of from each indicated group (n=5 per group) that were subjected to H&E staining. Original magnification: 20×. Steatohepatitis was scored by a histopathologist (who was blinded to the source condition of each sample) by a semiquantitative method derived from a published procedure [32]. (B) Liver function was assessed via the serum concentrations of triglycerides, cholesterol, ALT, and AST. (C) Transmission electron microscopy images of the liver in different experimental groups. Representative images at a magnification of 10,000× are shown. (D) Mitochondrial density and size. Mitochondrial densities were determined by normalizing the number of counted mitochondria to the area of each randomly selected cell (n=30 cells per group). Quantification of mitochondrial size was performed on five mice from each group. Data are shown as mean ± SEM, ^#P<0.05, ^##P<0.01, ^###P<0.001 vs the HF group; ^*P<0.05, ^**P<0.01, ^***P<0.001 vs the NF group according to ANNOVAS.