Research Paper
Circular RNA LPAR3 enhances GPX4 expression by targeting miR-196a-5p to suppress ferroptosis of osteosarcoma cells
Figure 6. CircLPAR3/miR-196a-5p/GPX4 axis is involved in regulating tumor growth in vivo. (A–E) Tumorigenicity assay in the nude mice (n = 5) analyzed the tumor growth of U2OS cells treated with control shRNA (shcontrol) or circLPAR3 shRNA. The tumor images (A), tumor volume (B), and weight (C) were presented. (D) The qPCR detected miR-196a-5p expression in the tumor tissues. (E) Western blot analysis determined GPX4 expression in the tumor tissues. The levels of lipid ROS (F) and iron (G) were analyzed by the corresponding measurement kits. ** P < 0.01.