Research Paper
Circular RNA LPAR3 enhances GPX4 expression by targeting miR-196a-5p to suppress ferroptosis of osteosarcoma cells
Figure 3. GPX4 participates in CircLPAR3-regulated ferroptosis in osteosarcoma cells. (A–D) The analysis was performed in the U2OS and MG63 cells treated with circLPAR3 shRNA and GPX4 reconstituted plasmid. (A) Western blot detected the GPX4 and SLC7A11 expression. The levels of iron (B), Fe2+ (C), and lipid ROS (D) were analyzed by the corresponding measurement kits. (E) The expression of SLC7A11 was measured in clinical osteosarcoma samples (n=50) by qPCR. (F) The expression of GPX4 was detected in clinical osteosarcoma samples (n=50) by qPCR. ** P < 0.01.