Research Paper

Figure 1. CircLPAR3 inhibition represses proliferation, invasion and migration but enhances apoptosis of osteosarcoma cells. (A) Heat map and hierarchical clustering analysis of circRNAs which were differentially expressed between clinical osteosarcoma samples (n=3) and primary chondroma samples (n=3) in circular RNA microarray analysis. Each column represented the expression profile of a tissue sample), and each row corresponds to a circRNA (log foldchange (FC) ≥ 2 and P < 0.05). Red color represented up-regulated circRNAs, and green color represented down-regulated circRNAs. (B) The expression of circLPAR3 was detected in clinical osteosarcoma samples (n=50) by qPCR. (C) The expression of circLPAR3 was determined by qPCR in hFOB1.19 cell line and osteosarcoma cell lines, including HOS, U2OS, 143B, MG63, and SJSA1. (D–I) The functional analysis was performed in the U2OS and MG63 cells treated with control shRNA (shcontrol) or circLPAR3 shRNA. (D) The expression of circLPAR3 was measured by qPCR in the cells. (E) The cell viability of U2OS and MG63 cells was analyzed by MTT assays. (F, G) The apoptosis of U2OS and MG63 cells was assessed by flow cytometry analysis. (H, I) The invasion and migration of U2OS and MG63 cells were detected by transwell assays. ** P < 0.01.