Research Paper Volume 14, Issue 11 pp 4699—4713

Hsa_circ_0063804 enhances ovarian cancer cells proliferation and resistance to cisplatin by targeting miR-1276/CLU axis


Figure 2. Circ_0063804 silencing promoted OC cells apoptosis and weakened proliferation and resistance to cisplatin. (A) Circ_0063804 expression in OC cells was successfully regulated via transfection. (B, C) According to CCK-8 assay and colony formation assay, circ_0063804 silencing inhibited OC cells proliferation, and circ_0063804 overexpression promoted OC cells proliferation. (D) Flow cytometry revealed that circ_0063804 silencing promoted OC cells apoptosis, and circ_0063804 overexpression inhibited OC cells apoptosis. (E) MTT assay indicated that circ_0063804 silencing reduced the IC50 of OC cells, and circ_0063804 overexpression elevated the IC50 of OC cells. (F) Circ_0063804 silencing reduced OC cells resistance to cisplatin, and circ_0063804 overexpression enhanced OC cells resistance to cisplatin. ** P < 0.01. CCK-8: cell counting kit-8. MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. OC: ovarian cancer.