Figure 2. The spread area, circularity and aspect ratio of cell and nucleus correlate with substrate rigidity. Spread area (A and B), circularity (C and D) and aspect ratio (E and F) of SKOV-3 cells and nucleus were analyzed using ImageJ software. Each column represents the means ± SE of 40-60 cells from 2 independent experiments. *P < 0.05, ***P <0.001, ****P < 0.0001.