Research Paper Volume 13, Issue 18 pp 22109—22119
LHX9, a p53-binding protein, inhibits the progression of glioma by suppressing glycolysis
Figure 3. Interfering with LHX9 expression accelerated the growth of glioma cells. (A) Interference with LHX9 expression in A172 and SK-N-SH cells. The target sequence was cloned into the pL.KO.1 vector, the virus was packaged to infect A172 and SK-N-SH cells. Cells were screened with puromycin for 1 week, and then the expression of LHX9 was identified. (B) The effect of interference with LHX9 expression on the growth of A172 and SK-N-SH cells was detected by CCK8 assay. (C, D) The effect of interference with LHX9 expression on the anchorage-independent growth of A172 and SK-N-SH cells was detected by soft agar assays. *, P<0.05; **, P<0.01.