Figure 4. miR-326 binds to and regulates FSCN1. (A) Predicted binding site of miR-326 and FSCN1 by the Target scan website. (B) Luciferase reporter assay was used to verify the binding of miR-326 and FSCN1. n=6. (C) RT-qPCR analysis of the level of FSCN1. n=6. (D) The expression of FSCN1 in ESCC and paracancerous tissues was detected by RT-PCR. n=6. (E) The correlation of FSCN1 and miR-326 in ESCC was analyzed by Pearson correlation analysis. (F) Knockdown of FSCN1 expression by siRNA in TE-1 cells inhibited the colony formation, wound healing, proliferation, invasion and increased cell apoptosis. N=3-4. * p < 0.05, ** p < 0.01.