Research Paper Volume 13, Issue 15 pp 19776—19788

Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis


Figure 3. lncRNA LINC01711 binds miR-326 as a sponge. (A) Possible secondary structures of LINC01711. (B) Linc01711 KEGG regulatory networks predicting by LnCAR. (C) The LINC01711 subcellular localization was verified by FISH. (D) Predicted binding site of LINC01711 and miR-326. (E) The expression of miR-326 in ESCC and paracancerous tissues was detected by RT-qPCR. n=6. (F) The correlation between the expression of LINC01711 and miR-326 in ESCC was analyzed by Pearson correlation analysis. (G) Dual luciferase reporter assay was used to verify the binding of LINC01711 and miR-326. n=6. (H) The binding of LINC01711 and Ago2 was measured by RIP method. n=3. (I) The enrichment of LINC01711 by miR-326 was detected by RNA pull-down. n=3. (J) The level of miR-326 in each group was measured by RT-qPCR. n=6. ** p < 0.01.