Figure 3. Microarray analysis exposed the regulation of the calcium ion pathway, and gene PI3KCA (PI3K) might influence the allicin-induced MI/R protection. (A) The hierarchical clustering analysis exhibited an intense distinction on differentially expressed mRNAs in MI/R+ Allicin as exhibited in the heat map. (B) The volcano plot exhibited an intense distinction on differentially expressed mRNAs in MI/R+ Allicin as exhibited in the heat map. (C) The qPCR analysis revealed that the level of PI3KCA(PI3K) was significantly decreased in MI/R+ Allicin tissues compared with I/R tissues. (P < 0.05, MI/R vs. Allicin). (D) The receiver operating characteristic (ROC) curve revealed that the expression level of PI3KCA is associated with a higher overall survival rate. (E) The annotations of Gene Ontology (GO) found 39 upregulated pathways. (F) The annotations of Gene Ontology (GO) found 37 downregulated pathways (