Figure 2. Allicin treatment reduced infarct size after M/IR and played the anti-apoptotic role in M/IR injury. (A) Masson staining to dye the heart of mice for evaluating the myocardial infarct area after the reperfusion, and the myocardial infarct size in the allicin group was fewer than that in the MI/R group. (B) The TUNEL staining, and the quantitative analysis revealed that very few TUNEL-positive cells were detected in the allicin group. (P < 0.05, Model vs. Allicin) (C) allicin treatment reduced Bax and cleaved caspase-3/9 protein expression and enhanced BCL-2 protein expression by western blot and the quantitative analysis. (P < 0.05, Model vs. Allicin).