Research Paper Volume 13, Issue 15 pp 19542—19560

Figure 5. Influence of heart salt RNAi, HSI, and LTE on the climbing ability and lifespan in Drosophila. (A) The climbing index in 1-week old, 3-week old, and 5-week old flies. (B) Fly population survival (%) curve. The leftmost curve represents the Salt-control+HSI group, of which flies had the shortest lifespan. (C) Influence of heart salt RNAi and HSI on climbing endurance in 1-week old flies. (D) Influence of heart salt RNAi and HSI on climbing endurance in 3-week old flies. (E) Influence of heart salt RNAi and HSI on climbing endurance in 5-week old flies. (F) Influence of heart salt RNAi, exercise, and HSI on climbing endurance in 1-week old flies. (G) Influence of heart salt RNAi, exercise, and HSI on climbing endurance in 3-week old flies. (H) Influence of heart salt RNAi, exercise, and HSI on climbing endurance in 5-week old flies. For climbing ability measurement, the sample size was about 100 flies for each group. For climbing index, using a one-way analysis of variance (ANOVA) followed by an LSD test among different groups. For lifespan, the sample size was 200-220 flies for each group. P-values for lifespan curves and climbing endurance curves were calculated by the log-rank test. Data are represented as means ± SEM. *P<0.05; **P <0.01.