Research Paper Volume 13, Issue 15 pp 19442—19459

Figure 6. CC16 secretion from CC16⁺ cells protects against DEP-induced bronchial epithelial cell inflammation via activation of autophagy. CC16⁺ cell conditioned medium containing different concentrations of CC16 is prepared, and used to culture BEAS-2B cells. The CC16 level in the medium of Ctrl, DEP, DEP+ CC16⁺, DEP+ DEP-CC16⁺ group is 0, 0, 144.7 and 46.5 ng/ml, respectively. After 12 h of culture, the cells and culture supernatants are collected. (A) LC3B and p62 protein expression in BEAS-2B cells are detected by western blot. (B) Autophagy induction is evaluated by LC3B immunostaining (× 400). Autophagosomes are identified as bright green dots. (C–E) TNF-α, IL-6, and IL-8 level in culture supernatants are detected by ELISA. ***P < 0.001 vs. ctrl; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs. DEP group; and ^&P < 0.05, ^&&P < 0.01, and ^&&&P < 0.001 vs. DEP+ CC16⁺ group.