**Figure 1.** **Evaluation of ITH with MATH in BCa.** (**A**) The workflow of this study. (**B**) The patients with high MATH values suffered an unfavorable prognosis. (**C**) The estimated MATH values acted as a potential predictor for chemosensitivity with Wilcoxon signer-rank test. (**D**) MATH values were positively correlated with TMB. (**E**) The cases in the high-MATH group had significantly lower immune and stromal components in TME. (**F**) Spearman correlation analysis indicated MATH values were negatively correlated with routine immune checkpoint genes, including *PD1, LAG3, GAL9, CTLA4, TIM-3,* and *TIGIT.* The red lines and green lines represented positive correlation and negative correlation, respectively. The boldness of the lines was positively associated with the strength of the correlation. ^{*}*p* < 0.05; ^{**}*p* < 0.01; ^{***}*p* < 0.001.