Figure 6. GTE-supplemented diet prevents the age-related loss of V0C interneurons. (A1, A2) A general view of a spinal cord hemisection of an adult mouse immunolabeled for VAChT (red) and counterstained with fluorescent Nissl (blue) for neuron identification; the arrow points to a V0C interneuron cluster located near the central canal (delimited by a dotted line); note also the different VAChT-positive MN pools in the ventral horn. (B) Density of V0C interneurons in spinal cords of aged animals from control and GTE groups; bars represent the mean ± SEM of 3-5 animals (20-28 images) per condition; *p < 0.05 vs. control (Student’s t-test); the red line indicates the mean value in adult mice found in [