Figure 4. Overexpression of THBS4 is correlated with vessel density in human GC tissues and predicts a poor prognosis. (A) The expression of THBS4 in a pan-cancer dataset. The red and green labels at the top represent high and low expression in certain tumor types, respectively. (B) Volcano plot revealed upregulation of THBS4 mRNA expression in GC compared with normal tissue. (C, D) The overall survival and disease-free survival analyses of THBS4 were plotted for patients with GC in TCGA. (E) Representative IHC staining with THBS4 antibody in GC and adjacent nontumor tissues. n= 5 samples per group. (F) Quantification of E. (G) Representative IHC staining with PECAM1 antibody in GC and adjacent nontumor tissues. n= 5 samples per group. (H) Quantification of G. (I) Correlation analysis of THBS4 and vessel density showed that THBS4 was positively correlated with vessel density in GC. (J) Validation of the positive correlation between THBS4 and PECAM1 in GEPIA.