Research Paper Volume 13, Issue 17 pp 21122—21133

Figure 4. ZNF609 is able to sponge miR-378b in glioma cells. (A) The binding site prediction of ZNF609 and miR-378b in ENCORI database. (B–D) The U251 and U87 cells were treated with miR-378b mimic. (B) The expression of miR-378b was detected by qPCR. (C and D) The luciferase activity was detected by dual luciferase reporter assays. (E–F) The interaction of ZNF609 with miR-378b by RIP assays. [26] The expression of miR-378b was measured by qPCR in U251 and U87 cells treated with ZNF609 siRNA. mean ± SD, ^**P < 0.01.