Research Paper Volume 13, Issue 14 pp 17978—18005

Figure 1. Reduced expression of HDACs expression in replicative senescence. (A, B) Representative Western blots showing HDACs 1-7 and p16^INK-4A protein level in young (early passage, Y), intermediate (I) or replicative senescent (RS) AG04431 cells (A) or primary normal human dermal fibroblasts (NHDFs) isolated from an adult donor (57 years) or a young donor (3 years) (B). (C, D) Quantifications of the protein level of HDACs 1-7 and p16 ^INK-4A, with α-tubulin (AG04431, n=4) or GAPDH (NHDFs, n=2) as loading control. (E, F) Class IIa HDACs activity in AG04431 (e) (n=3) or in NHDFs (F) (n=3). Statistical analyses were performed using a t-test (*: p<0.05).