Research Paper Volume 13, Issue 14 pp 18498—18514

Figure 7. M/T-Exo miRNA-205 enhances the tamoxifen resistance in vivo. (A) Tumor growth curves of mice injected with the negative control miRNA-205 inhibitor (NCi), miRNA-205 inhibitor (205i), or lentiviral vector carrying E2F1 (E2F1). (B) Tumor growth curves of mice injected with M/T-Exo when the tumor size was around 50 mm³. (C) Tumor growth curves of mice injected with tamoxifen when the tumor size was around 100 mm³. (D). Representative images of the tumor. (E) After all treatments, the expression of miRNA-205 in tumors of mice on day 9 post-tamoxifen treatment. (F–G). The mRNA and protein expressions of E2F1 in tumors of mice on day 9 post-tamoxifen treatment. (H). The protein expressions of cleaved caspase-9 and caspase-3, Akt, and the phosphorylation of Akt at Ser 473 (p-Akt Ser 473) in tumors of mice on day 9 post-tamoxifen treatment. Values are means ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. At least three replicates were available for analysis in each treatment group.