Research Paper Volume 13, Issue 15 pp 19306—19316

LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p

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Figure 5. HAGLR promoted TNBC growth through miR-335-3p/WNT2 axis. HAGLR was transfected into BT549 cells with miR-335-3p or si-WNT2. (A) The transfection efficiency was detected using qRT-PCR. (B) CCK8 assay for cell viability of BT549 cells. (C) EdU assay for cell proliferation of BT549 cells. Scale bar, 100 μm. (D) Wound healing assay for cell migration of BT549 cells. Scale bar, 100 μm. (E) Transwell assay for cell invasion of BT549 cells. Scale bar, 50 μm. Data are mean ± SD; *P < 0.05 vs pcDNA3.1, #P < 0.05 vs HAGLR.