Figure 1. Characterization and concentration analysis of extracellular vesicles. (A) Plasma-derived EVs isolated from four individuals with osteoporosis, four individuals without osteoporosis, one EV-depleted plasma sample were subjected to SDS-PAGE and probed for EV-enriched proteins, Annexin-V and TSG101. (B) Electron microscopy of EVs. Scale bar = 500 nm. (C) and (D) Nanoparticle tracking analysis (NTA). The area under the curve in (C) is shown in (D).