Research Paper Volume 13, Issue 13 pp 17568—17591

Aging-dependent loss of GAP junction proteins Cx46 and Cx50 in the fiber cells of human and mouse lenses accounts for the diminished coupling conductance

class="figure-viewer-img"

Figure 10. Differential expression patterns of the connexins Cx43, Cx46 and Cx50 in the epithelial cells of the αA-/- and αB-/- mouse lenses (Figure 10A10C), and in the fiber cells of the αA-/- mouse lenses (Figure 10D10E). (A) Western blot analysis of Cx43 and Cx50 in the lens epithelial cells of the αA-/- and αB-/- mice. β-Tubulin was showed as a loading control. (B) & (C) Quantification of the western blot results in (A). (D) Western blot analysis of Cx43 and Cx50 in the lens fiber cells of the αA-/- mice. α-Actinin was showed as a loading control. (E) Quantification of the western blot results in (D). NS, not significant, *p < 0.05, **p < 0.01, ***p < 0.001.