Research Paper Volume 13, Issue 15 pp 19243—19259

Glioma stem cell-derived exosomal miR-944 reduces glioma growth and angiogenesis by inhibiting AKT/ERK signaling

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Figure 6. Exosomal miR-944 derived from GSCs suppresses angiogenesis of HUVECs. (A, B) Tube formation assay results show the number of branch points as an index of angiogenesis in HUVECs co-cultured with GSC/agomiR-NC-Exo and GSC/agomiR-944-Exo for 24 h. (CG) Western blot analysis shows levels of (C, D) VEGF, (C, E) angiogenin-1, (C, F) MMP9, and (C, G) MMP14 in HUVECs co-cultured with GSC/agomiR-NC-Exo and GSC/agomiR-944-Exo for 24 h. β-actin was used as an internal control. **P < 0.01 vs. the GSC/agomiR-NC-Exo group. GSCs, glioma stem cells; HUVECs, human umbilical vein endothelial cells.