Research Paper Volume 14, Issue 11 pp 4673—4698

Figure 8. Further ex vivo IF examining the expression of CXCR4, EMT- and CSC-related proteins in the OVCA420 and SKOV3 cell xenograft tissues from the tumours nude mice following treatment with PTX. Immunofluorescent (IF) staining analysis images illustrated the location of CXCR4, EMT-, and CSC-related protein expressions in the OVCA420 (A) and SKOV3 (B) cell xenograft tumour tissues. Notably, red fluorescence shows the membrane expression of CXCR4, snail, CD44, and CD133; green fluorescence shows the membrane expression of E-cadherin, vimentin, and NANOG; and blue fluorescence shows all cell nuclei stained with DAPI (4′, 6-diamidino-2-phenylindole). Data represent one of the three independent experiments with similar results. Scale bars=50 μm.