Research Paper Volume 13, Issue 14 pp 18223—18237

Figure 3. Effects of combination treatment with RAPA and SAHA on DNA damage and repair after IR in NSCLC cells. (A, B) the protein level of γ-H2AX was determined by western blot analysis. Two NSCLC cells (A549, SK-MES-1) were treated with RAPA (100nmol/L) or/and SAHA (2.5μmol/L) for 24h and were subsequently exposed to IR (4Gy), the γ-H2AX protein was tested at 1h and 24h after IR. *p<0.05, 1h after IR versus control; ^#p<0.05, 24h versus 1h after IR; ⁺p<0.05, combined treatment versus single treatment 24h after IR. (C–F) the protein (C) and mRNA level of Rad51 (D), Ku80 (E), Ku70 (F) were determined by western blot and RT-qPCR analysis. Two NSCLC cells (A549, SK-MES-1) were treated with RAPA (100nmol/L) or/and SAHA (2.5μmol/L) for 24h and were subsequently exposed to IR (4Gy) and were tested after 4h. *p<0.05, compared with control, ^#p<0.05, compared with IR.