Research Paper Volume 13, Issue 12 pp 15917—15941

Figure 4. PDS downregulates Brca1 in primary neurons but not in primary astrocytes and microglial cells. (A) Primary cortical neurons, astrocytes and microglial cells were treated with a vehicle or with 2 μM PDS overnight (18 h). Neurons were collected and lysed, and Brca1 expression was determined by qRT-PCR normalized to Tbp. *p=0.05 (t=4.304> t_0.975,2 = 4.303; significant). Results were pooled from duplicates of 2–4 independent reactions. (B) Primary cortical neurons, astrocytes and microglial cells were treated with a vehicle or with 2 μM etoposide overnight (18 h). Cells were collected and lysed, and Brca1 expression was determined by qRT-PCR normalized to Tbp. *p<0.038, ****p< 0.0001 (t-test). Results were pooled from duplicates of 2–4 independent reactions.