Research Paper

Figure 9. Overexpressing miR-15b-5p facilitated gastric cancer cell proliferation and tumor-associated macrophages invasion in vivo. 5×10⁶ AGS human gastric carcinoma cells were injected subcutaneously in each mouse at the right side, and miR-15b-5p-mimics or miR-NC were directly injected into the xenograft tumor at a dose of 10⁶ cells/50μL, with eight times over three days. After injection, the mice were anesthetized on day 5, 10, 15, 20 and 25, respectively, to collect the tumors. (A–C) The tumor volume and weight. (D) QRT-PCR was implemented to testify the profiles of molecular markers (MMP1,2,9, IL-4, IL-10, and VEGF) of TAMs. (E) The expression of TAM polarization markers (iNOS, CD68, CD163, and CD20) of TAMs was monitored by WB. NsP > 0.05, *P < 0.05, **P < 0.01, * * * P < 0.001, (vs miR - NC group), N = 5.