Research Paper
Exosomal miR-15b-5p derived from gastric cancer cells enhances immune infiltrating of tumor-associated macrophages through the WIF1/WNT5A axis
Figure 8. The inhibition of miR-15b-5p or WNT5A in tumor-associated macrophages weakens their tumor-promoting effect in AGS cells. (A) The cell processing method. MiR-15b-5p inhibitor or WNT5A inhibitor was transfected into TAMs, which were then treated with exosomes of GC cells with overexpressing miR-15b-5p. Then the TAMs were coculture with AGS cells or VECs. (B, C) AGS human gastric carcinoma cells proliferation and apoptosis were testified by CCK-8 assay and flow cytometry, respectively. (D) Transwell assay was applied to detect GC cell migration and invasion. (E) VEC proliferation was measured by the CCK-8 method. (F) Tube formation assay was employed to verify VEC angiogenesis. (G) Protein profiles of VEGFA, FGF and PI3k/Akt/eNOS in VECs were compared by WB. *P < 0.05, **P<0.01, ***P<0.001 (vs.TAMs+miR-NC-in+ exomiR-15b-5p group), N=3.