Research Paper
Exosomal miR-15b-5p derived from gastric cancer cells enhances immune infiltrating of tumor-associated macrophages through the WIF1/WNT5A axis
Figure 7. Inhibiting miR-15b-5p or WNT5A in the tumor-associated macrophages reversed "M2" phenotype alteration. miR-15b-5p inhibitor or WNT5A inhibitor was transfected into TAMs, which were then treated with exosomes of GC cells with overexpressing miR-15b-5p. (A) The miR-15b-5p expression in TAMs was monitored by qRT-PCR. (B) qRT-PCR was carried out to examine the profiles of molecular markers (MMP1, MMP2, MMP9, IL-4, IL-10, and VEGF) of TAMs. (C–E) WB was applied to test the expression of molecular markers (iNOS, CD68, CD163, and CD20) and WIF1/WNT5A of TAMs. * P < 0.05, * *P < 0.01, * * * P < 0.001, (vs miR-NC-in+exomiR-15b-5p group), N = 3.