← Back
Research Paper

Exosomal miR-15b-5p derived from gastric cancer cells enhances immune infiltrating of tumor-associated macrophages through the WIF1/WNT5A axis

class="figure-viewer-img"

Figure 6. miR-15b-5p inhibition in tumor-associated macrophages attenuated the malignant phenotypes of gastric cancer cells and angiogenesis of vascular endothelial cells. (A) The cell processing method. (B, C) AGS human gastric carcinoma cells proliferation and apoptosis were testified by CCK-8 and flow cytometry, respectively. (D) Transwell assay was applied to detect GC cell migration and invasion. (E) VEC proliferation was measured by the CCK-8 method. (F) Tube formation assay was employed to verify VEC angiogenesis. (G) Protein profiles of VEGFA, FGF and PI3k/Akt/eNOS in VEC were compared by WB. **P<0.01, ***P<0.001 (vs.TAMs+ EXOmiR-NC-in group), N=3.