Research Paper
Exosomal miR-15b-5p derived from gastric cancer cells enhances immune infiltrating of tumor-associated macrophages through the WIF1/WNT5A axis
Figure 5. Gastric cancer cell-derived exosomes knocking down miR-15b-5p weakened the "M2" polarization. A miR-15b-5p knockdown model was constructed in GC cells co-cultured with THP-1 monocytes. (A) The miR-15b-5p expression was monitored by qRT-PCR. (B) QRT-PCR was carried out to examine the profiles of molecular markers (MMP1, MMP2, MMP9, IL-4, IL-10, and VEGF) of TAMs. (C–E) WB was applied to test the expression of molecular markers (iNOS, CD68, CD163, and CD20) of TAMs and WIF1/WNT5A. * P < 0.05, * *P < 0.01, * * * P < 0.001, (vs GC + miR-NC-in group), N = 3.