Research Paper
Exosomal miR-15b-5p derived from gastric cancer cells enhances immune infiltrating of tumor-associated macrophages through the WIF1/WNT5A axis
Figure 4. Tumor-associated macrophages overexpressing miR-15b-5p deteriorated the malignant phenotype of gastric cancer cells and angiogenesis of vascular endothelial cells. (A) The cell processing method. (B, C) AGS human gastric carcinoma cells proliferation and apoptosis were monitored by CCK-8 and flow cytometry, respectively. (D) Transwell assay was applied to verify GC cell migration and invasion. (E) VEC proliferation was measured by the CCK-8 method. (F) Tube formation assay was implemented to examine VEC angiogenesis. (G) Protein profiles of VEGFA, FGF and PI3k/Akt/eNOS were compared by WB.*P<0.05, **P<0.01, ***P<0.001 (vs.TAMs+EXOmiR-NC group), N=3.