Research Paper
Exosomal miR-15b-5p derived from gastric cancer cells enhances immune infiltrating of tumor-associated macrophages through the WIF1/WNT5A axis
Figure 3. Gastric cancer cell-derived exosomes overexpressing miR-15b-5p accelerated the "M2" polarization of tumor-associated macrophages. miR-15b-5p mimics were transfected into GC cells co-cultured with THP-1 monocytes. (A) The miR-15b-5p level was detected by qRT-PCR. (B) QRT-PCR was carried out to verify the levels of molecular markers (MMP1, MMP2, MMP9, IL-4, IL-10, and VEGF) of TAMs. (C–E) WB was conducted to examine the expression of molecular markers (iNOS, CD68, CD163, and CD20) of TAMs and the WIF1/WNT5A pathway. *P<0.05, **P<0.01, ***P<0.001 (vs.GC+ miR-NC group), N=3.