Research Paper
Exosomal miR-15b-5p derived from gastric cancer cells enhances immune infiltrating of tumor-associated macrophages through the WIF1/WNT5A axis
Figure 2. Tumor-associated macrophages strengthened the malignant phenotypes of gastric cancer cells and angiogenesis of vascular endothelial cells. (A) The cell processing method. (B, C) AGS human gastric carcinoma cells proliferation and apoptosis were detected by CCK-8 and flow cytometry, respectively. (D) Transwell assay was employed to determine the influence of TAMs on GC cell migration and invasion. (E) CCK-8 method was conducted to determine the impact of TAMs on VEC proliferation. (F) Tube formation assay was employed to test VEC angiogenesis. (G) The protein expression VEGFA, FGF, and PI3k/Akt/eNOS were compared by WB. **P<0.01, ***P<0.001 (vs. Con group), N=3.